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gen5 2.01.14 data analysis software  (Agilent technologies)


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    Structured Review

    Agilent technologies gen5 2.01.14 data analysis software
    Gen5 2.01.14 Data Analysis Software, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gen5+software/pm39163813-220-7-12
    Average 90 stars, based on 1 article reviews
    gen5 2.01.14 data analysis software - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Anticancer Effects of Spiperone in C57BL/6 Mice with Emphysema and Lung Carcinoma.
    Article Snippet: The antitumor and antimetastatic activity of dopamine D2 receptor antagonists spiperone was studied in C57BL/6 mice in a model of combined pathology (emphysema and lung cancer).. Emphysema was induced by administration of LPS and cigarette smoke extract.. Lung cancer was induced by injection of Lewis lung carcinoma cells into the lung.

    Article Title: Fibroblast growth receptor 1 is regulated by G-quadruplex in metastatic breast cancer
    Article Snippet: Paraffin sectioning at 5μm thickness and H&E staining were conducted by AML laboratories, Inc. (Jacksonville, FL). .. The images of lung sections were obtained by Cytation 5 cell imaging multi-mode reader with Gen5 software (BioTek Instruments, Inc.). .. All in vivo assays have complied with all relevant ethical regulations for animal use were conducted under IACUC approval from Purdue University.

    Software:

    Article Title: Anticancer Effects of Spiperone in C57BL/6 Mice with Emphysema and Lung Carcinoma.
    Article Snippet: The antitumor and antimetastatic activity of dopamine D2 receptor antagonists spiperone was studied in C57BL/6 mice in a model of combined pathology (emphysema and lung cancer).. Emphysema was induced by administration of LPS and cigarette smoke extract.. Lung cancer was induced by injection of Lewis lung carcinoma cells into the lung.

    Article Title: Macrophage-Induced Pro-Fibrotic Gene Expression in Tubular Cells after Ischemia/Reperfusion Is Paralleled but Not Directly Mediated by C5a/C5aR1 Signaling.
    Article Snippet: Cell culture supernatants were analyzed for TGF-ß using a human TGF-ß ELISA kit (R&D System , Mi neapolis, MN, USA) f llowing the m nufacturer’s instructions and using a Synergy microplate reader and Gen5 software (BioTek In truments GmbH, Fr edrichshall, Germany). .. Rat plasma samples collected at 10 min, 6 h, 24 h, 3 days, 5 days and 8 weeks after I/R were analyzed for C5a using a rat complement component C5a ELISA kit (NBP2-82137, Novus Biologicals, Centennial, CO, USA) following the manufacturer’s instructions and using a Synergy microplate reader and Gen5 software (BioTek Instruments GmbH, Friedrichshall, Germany). ..

    Article Title: The parkin V380L variant is a genetic modifier of Machado-Joseph disease with impact on mitophagy.
    Article Snippet: Expression was terminated by the addition of doxycycline (Merck; 4.5 μM) at desired time points before cell harvest. .. Assessment of cell viability was performed as previously described [36] with the following specifications: 5,000 cells/well were seeded in 96-well cell culture plates (ViewPlate-96 Black, Perkin Elmer, Massachusetts, USA) and transfected 24 h later using Turbofectin 8.0 for 72 h. Cells were treated with 12.5 μM CCCP or vehicle control DMSO for the last 24 h. Afterwards, culture medium was aspirated, cells incubated in fresh medium containing the resazurinbased PrestoBlueTM Cell Viability Reagent (Thermo Fisher Scientific) in a 1:10 ratio under standard culture conditions for 60 min. Fluorescence signals were measured at 535 nm (excitation)/615 nm (emission) using a Synergy HT plate reader and the Gen5 software (both BioTek Instruments, Winooski, VT, USA). ..

    Article Title: IL-1β-activated PI3K/AKT and MEK/ERK pathways coordinately promote induction of partial epithelial–mesenchymal transition
    Article Snippet: Imaging of the wound was performed every hour for 48 h using a Lionheart FX Automated Live Cell Imager (BioTek Instruments, Winooski, VT). .. Quantification of wound confluence was performed using Gen5 software (BioTek Instruments). .. Kinase phosphorylation in A549 cells treated with IL-1β was determined using a Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems) following the manufacturer’s instructions.

    Article Title: Characterization of novel double-reporter strains of Mycobacterium abscessus for drug discovery: a study in mScarlet.
    Article Snippet: The acquisition was performed in a BD LSRFortessa Cell Analyser (BD Biosciences) using the 610/20 nm filter, and data analysis was performed in the FlowJo software (BD Biosciences). .. Luminescence stability in liquid culture To address the stability of the luminescent signal, exponential phase cultures of Mab operon_mScarlet and Mab FF_mScarlet were plated in 96-well white plates (Thermo Scientific), and the luminescent signal was acquired in 3-min intervals (integration time = 0.1 s) over 30 min using a Synergy Mx microplate reader and the Gen5 software (BioTek, Agilent Technologies). .. In the case of Mab FF_mScarlet, IVISbrite D-luciferin potassium salt (PerkinElmer) at different concentrations in H2O was added to the wells (10%, vol/ vol).

    Article Title: Discovery of Potent Isoquinolinequinone N -Oxides to Overcome Cancer Multidrug Resistance
    Article Snippet: Following washing steps in acetic acid, the bound dye was solubilized with 10 mM Tris base solution (Merck Life Science). .. Absorbance was measured at 510 nm in a multiplate reader (Synergy Mx, Biotek Instruments Inc., Winooski, Vermont, USA) using the Gen5 software (Biotek Instruments Inc.). ..

    Article Title: BAX and DDB2 as biomarkers for acute radiation exposure in the human blood ex vivo and non-human primate models.
    Article Snippet: .. The plates were read using BioTek Synergy H1 Multimode Microplate Reader (Agilent Technologies, Santa Clara, CA) and analyzed using the built-in Gen5 software. .. Optical density readings were then interpreted using GainData®, Arigo Biolaboratories’ online calculator to plot standard curves and interpolate unknown concentrations.

    Article Title: Fibroblast growth receptor 1 is regulated by G-quadruplex in metastatic breast cancer
    Article Snippet: Paraffin sectioning at 5μm thickness and H&E staining were conducted by AML laboratories, Inc. (Jacksonville, FL). .. The images of lung sections were obtained by Cytation 5 cell imaging multi-mode reader with Gen5 software (BioTek Instruments, Inc.). .. All in vivo assays have complied with all relevant ethical regulations for animal use were conducted under IACUC approval from Purdue University.

    Clinical Proteomics:

    Article Title: Macrophage-Induced Pro-Fibrotic Gene Expression in Tubular Cells after Ischemia/Reperfusion Is Paralleled but Not Directly Mediated by C5a/C5aR1 Signaling.
    Article Snippet: Cell culture supernatants were analyzed for TGF-ß using a human TGF-ß ELISA kit (R&D System , Mi neapolis, MN, USA) f llowing the m nufacturer’s instructions and using a Synergy microplate reader and Gen5 software (BioTek In truments GmbH, Fr edrichshall, Germany). .. Rat plasma samples collected at 10 min, 6 h, 24 h, 3 days, 5 days and 8 weeks after I/R were analyzed for C5a using a rat complement component C5a ELISA kit (NBP2-82137, Novus Biologicals, Centennial, CO, USA) following the manufacturer’s instructions and using a Synergy microplate reader and Gen5 software (BioTek Instruments GmbH, Friedrichshall, Germany). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Macrophage-Induced Pro-Fibrotic Gene Expression in Tubular Cells after Ischemia/Reperfusion Is Paralleled but Not Directly Mediated by C5a/C5aR1 Signaling.
    Article Snippet: Cell culture supernatants were analyzed for TGF-ß using a human TGF-ß ELISA kit (R&D System , Mi neapolis, MN, USA) f llowing the m nufacturer’s instructions and using a Synergy microplate reader and Gen5 software (BioTek In truments GmbH, Fr edrichshall, Germany). .. Rat plasma samples collected at 10 min, 6 h, 24 h, 3 days, 5 days and 8 weeks after I/R were analyzed for C5a using a rat complement component C5a ELISA kit (NBP2-82137, Novus Biologicals, Centennial, CO, USA) following the manufacturer’s instructions and using a Synergy microplate reader and Gen5 software (BioTek Instruments GmbH, Friedrichshall, Germany). ..

    Cell Culture:

    Article Title: The parkin V380L variant is a genetic modifier of Machado-Joseph disease with impact on mitophagy.
    Article Snippet: Expression was terminated by the addition of doxycycline (Merck; 4.5 μM) at desired time points before cell harvest. .. Assessment of cell viability was performed as previously described [36] with the following specifications: 5,000 cells/well were seeded in 96-well cell culture plates (ViewPlate-96 Black, Perkin Elmer, Massachusetts, USA) and transfected 24 h later using Turbofectin 8.0 for 72 h. Cells were treated with 12.5 μM CCCP or vehicle control DMSO for the last 24 h. Afterwards, culture medium was aspirated, cells incubated in fresh medium containing the resazurinbased PrestoBlueTM Cell Viability Reagent (Thermo Fisher Scientific) in a 1:10 ratio under standard culture conditions for 60 min. Fluorescence signals were measured at 535 nm (excitation)/615 nm (emission) using a Synergy HT plate reader and the Gen5 software (both BioTek Instruments, Winooski, VT, USA). ..

    Transfection:

    Article Title: The parkin V380L variant is a genetic modifier of Machado-Joseph disease with impact on mitophagy.
    Article Snippet: Expression was terminated by the addition of doxycycline (Merck; 4.5 μM) at desired time points before cell harvest. .. Assessment of cell viability was performed as previously described [36] with the following specifications: 5,000 cells/well were seeded in 96-well cell culture plates (ViewPlate-96 Black, Perkin Elmer, Massachusetts, USA) and transfected 24 h later using Turbofectin 8.0 for 72 h. Cells were treated with 12.5 μM CCCP or vehicle control DMSO for the last 24 h. Afterwards, culture medium was aspirated, cells incubated in fresh medium containing the resazurinbased PrestoBlueTM Cell Viability Reagent (Thermo Fisher Scientific) in a 1:10 ratio under standard culture conditions for 60 min. Fluorescence signals were measured at 535 nm (excitation)/615 nm (emission) using a Synergy HT plate reader and the Gen5 software (both BioTek Instruments, Winooski, VT, USA). ..

    Control:

    Article Title: The parkin V380L variant is a genetic modifier of Machado-Joseph disease with impact on mitophagy.
    Article Snippet: Expression was terminated by the addition of doxycycline (Merck; 4.5 μM) at desired time points before cell harvest. .. Assessment of cell viability was performed as previously described [36] with the following specifications: 5,000 cells/well were seeded in 96-well cell culture plates (ViewPlate-96 Black, Perkin Elmer, Massachusetts, USA) and transfected 24 h later using Turbofectin 8.0 for 72 h. Cells were treated with 12.5 μM CCCP or vehicle control DMSO for the last 24 h. Afterwards, culture medium was aspirated, cells incubated in fresh medium containing the resazurinbased PrestoBlueTM Cell Viability Reagent (Thermo Fisher Scientific) in a 1:10 ratio under standard culture conditions for 60 min. Fluorescence signals were measured at 535 nm (excitation)/615 nm (emission) using a Synergy HT plate reader and the Gen5 software (both BioTek Instruments, Winooski, VT, USA). ..

    Incubation:

    Article Title: The parkin V380L variant is a genetic modifier of Machado-Joseph disease with impact on mitophagy.
    Article Snippet: Expression was terminated by the addition of doxycycline (Merck; 4.5 μM) at desired time points before cell harvest. .. Assessment of cell viability was performed as previously described [36] with the following specifications: 5,000 cells/well were seeded in 96-well cell culture plates (ViewPlate-96 Black, Perkin Elmer, Massachusetts, USA) and transfected 24 h later using Turbofectin 8.0 for 72 h. Cells were treated with 12.5 μM CCCP or vehicle control DMSO for the last 24 h. Afterwards, culture medium was aspirated, cells incubated in fresh medium containing the resazurinbased PrestoBlueTM Cell Viability Reagent (Thermo Fisher Scientific) in a 1:10 ratio under standard culture conditions for 60 min. Fluorescence signals were measured at 535 nm (excitation)/615 nm (emission) using a Synergy HT plate reader and the Gen5 software (both BioTek Instruments, Winooski, VT, USA). ..

    Fluorescence:

    Article Title: The parkin V380L variant is a genetic modifier of Machado-Joseph disease with impact on mitophagy.
    Article Snippet: Expression was terminated by the addition of doxycycline (Merck; 4.5 μM) at desired time points before cell harvest. .. Assessment of cell viability was performed as previously described [36] with the following specifications: 5,000 cells/well were seeded in 96-well cell culture plates (ViewPlate-96 Black, Perkin Elmer, Massachusetts, USA) and transfected 24 h later using Turbofectin 8.0 for 72 h. Cells were treated with 12.5 μM CCCP or vehicle control DMSO for the last 24 h. Afterwards, culture medium was aspirated, cells incubated in fresh medium containing the resazurinbased PrestoBlueTM Cell Viability Reagent (Thermo Fisher Scientific) in a 1:10 ratio under standard culture conditions for 60 min. Fluorescence signals were measured at 535 nm (excitation)/615 nm (emission) using a Synergy HT plate reader and the Gen5 software (both BioTek Instruments, Winooski, VT, USA). ..



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